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Please use this identifier to cite or link to this item: https://repository.monashhealth.org/monashhealthjspui/handle/1/37350
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dc.contributor.authorArnau G.M.en
dc.contributor.authorCowley K.J.en
dc.contributor.authorSemple T.en
dc.contributor.authorShortt, Jakeen
dc.contributor.authorSimpson K.J.en
dc.contributor.authorCluse L.A.en
dc.contributor.authorNikolic I.en
dc.contributor.authorKnight D.en
dc.contributor.authorJohnstone R.W.en
dc.contributor.authorMadhamshettiwar P.B.en
dc.contributor.authorLuu J.en
dc.date.accessioned2021-05-14T12:42:21Zen
dc.date.available2021-05-14T12:42:21Zen
dc.date.created20180125en
dc.date.issued2018en
dc.identifier.citationMethods in Molecular Biology. 1725 (pp 201-227), 2018. Date of Publication: 2018.en
dc.identifier.issn1064-3745en
dc.identifier.urihttps://repository.monashhealth.org/monashhealthjspui/handle/1/37350en
dc.description.abstractThis chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen-with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories.Copyright © 2018, Springer Science+Business Media, LLC.en
dc.languageEnglishen
dc.languageenen
dc.publisherHumana Press Inc. (E-mail: humana@humanapr.com)en
dc.relation.ispartofMethods in Molecular Biologyen
dc.titleA comprehensive protocol resource for performing pooled shRNA and CRISPR screens.en
dc.typeChapteren
dc.typeChapter-
dc.identifier.doihttp://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1007/978-1-4939-7568-6_17en
dc.publisher.placeUnited Statesen
dc.identifier.pubmedid29322420 [http://www.ncbi.nlm.nih.gov/pubmed/?term=29322420]en
dc.identifier.source620283936en
dc.identifier.institution(Cluse, Knight, Johnstone) Gene Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia (Nikolic, Madhamshettiwar, Luu, Cowley, Simpson) Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia (Semple, Arnau) Molecular Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia (Shortt) School of Clinical Sciences at Monash Health, Monash University, Clayton, VIC, Australia (Johnstone, Simpson) Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, Australiaen
dc.description.addressK.J. Simpson, Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, Australia. E-mail: Kaylene.simpson@petermac.orgen
dc.description.publicationstatusEmbaseen
dc.rights.statementCopyright 2018 Elsevier B.V., All rights reserved.en
dc.subect.keywordsDrop out screen Lentivirus Pooled CRISPR Pooled shRNAen
dc.identifier.authoremailSimpson K.J.; Kaylene.simpson@petermac.orgen
dc.description.grantOrganization: (ACRF) *Australian Cancer Research Foundation* Organization No: 501100000947 Country: Australia Organization: *Cancer Council Victoria* Organization No: 501100000951 Country: Australia Organization: *Leukaemia Foundation* Organization No: 501100000926 Country: Australia Organization: (NHMRC) *National Health and Medical Research Council* Organization No: 501100000925 Country: Australia Organization: (VCA) *Victorian Cancer Agency* Organization No: 100008018 Country: Australiaen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairetypeChapter-
item.openairetypeChapter-
item.cerifentitytypePublications-
item.cerifentitytypePublications-
crisitem.author.deptHaematology-
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