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Please use this identifier to cite or link to this item: https://repository.monashhealth.org/monashhealthjspui/handle/1/26770
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dc.contributor.authorHuang J.en
dc.contributor.authorTeh B.M.en
dc.contributor.authorLi H.en
dc.contributor.authorHu Y.en
dc.contributor.authorShen Y.en
dc.contributor.authorZheng M.en
dc.contributor.authorBu S.en
dc.contributor.authorLv C.en
dc.contributor.authorYao X.en
dc.date.accessioned2021-05-14T08:54:15Zen
dc.date.available2021-05-14T08:54:15Zen
dc.date.copyright2021en
dc.date.created20210406en
dc.date.issued2021-04-06-
dc.date.issued2021-04-06en
dc.identifier.citationOtolaryngology - Head and Neck Surgery (United States). 164 (2) (pp 381-390), 2021. Date of Publication: February 2021.en
dc.identifier.issn0194-5998en
dc.identifier.urihttps://repository.monashhealth.org/monashhealthjspui/handle/1/26770en
dc.description.abstractObjective: To evaluate the efficacy of acellular collagen scaffold (ACS) in combination with basic fibroblast growth factor (bFGF) for the repair of traumatic tympanic membrane (TM) perforation in a rat model. Study Design: A prospective controlled animal study in a rat model of traumatic TM perforation. Setting(s): Tertiary medical center. Subjects and Methods: Sprague-Dawley rats (N = 84) with unilateral traumatic perforation of the right TMs were randomized to receive ACS, bFGF, ACS in combination with bFGF (ACS/bFGF), or nothing (spontaneous healing without any interventions as a control group). The healing outcomes were evaluated by otoscopy, optical coherence tomography, histology, and transmission electron microscopy at 1, 2, and 4 weeks postoperatively. The hearing outcomes were assessed with auditory brainstem response testing. Result(s): ACS/bFGF resulted in higher perforation closure rates at an earlier stage than spontaneous healing, ACS, and bFGF. Based on histology, optical coherence tomography, and transmission electron microscopy, a trilaminar structure and uniform thickness with mature, densely packed collagen fibers were seen in the ACS/bFGF group. Auditory brainstem response evaluation also showed that ACS/bFGF treatment promoted faster functional hearing recovery as compared with the control group. Conclusion(s): ACS is an effective TM scaffold and a carrier for bFGF. ACS/bFGF improves the TM closure rate, results in better-reconstructed TMs, and improves hearing. ACS/bFGF serves as a potential substitute for TM perforations in clinical settings.Copyright © American Academy of Otolaryngology-Head and Neck Surgery Foundation 2020.en
dc.languageenen
dc.languageEnglishen
dc.publisherSAGE Publications Inc.en
dc.relation.ispartofOtolaryngology - Head and Neck Surgeryen
dc.subjecttissue regenerationen
dc.subjecttransmission electron microscopyen
dc.subjecttympanometryen
dc.subjecttympanoplastyen
dc.subjectultrastructureen
dc.subjectwound healingen
dc.subject*collagenen
dc.subjectcollagen type 1en
dc.subjectfibroblast growth factoren
dc.subjectfibroblast growth factor 2en
dc.subject*molecular scaffolden
dc.subjectotoscopeen
dc.subjectModel 4000en
dc.subjectTECNAI 10en
dc.subject*perforationen
dc.subjectanimal experimenten
dc.subjectanimal modelen
dc.subjectanimal tissueen
dc.subjectarticleen
dc.subjectbrain stem responseen
dc.subjectcontrolled studyen
dc.subjectevoked brain stem auditory responseen
dc.subjectevoked brain stem responseen
dc.subject*fibroblasten
dc.subjecthearingen
dc.subjecthistologyen
dc.subjecthistopathologyen
dc.subjectimmunohistochemistryen
dc.subjectmaleen
dc.subjectmyringoplastyen
dc.subjectnonhumanen
dc.subjectoptical coherence tomographyen
dc.subjectotoscopyen
dc.subjectprospective studyen
dc.subjectraten
dc.subjectscanning electron microscopyen
dc.titleAcellular Collagen Scaffold With Basic Fibroblast Growth Factor for Repair of Traumatic Tympanic Membrane Perforation in a Rat Model.en
dc.typeArticleen
dc.identifier.doihttp://monash.idm.oclc.org/login?url=-
dc.identifier.doihttp://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1177/0194599820938345en
dc.publisher.placeUnited Statesen
dc.identifier.pubmedid32662734 [http://www.ncbi.nlm.nih.gov/pubmed/?term=32662734]en
dc.identifier.source2005572502en
dc.identifier.institution(Yao, Li, Hu, Huang, Lv, Shen) Department of Otolaryngology-Head and Neck Surgery, Ningbo Medical Center, Ningbo Lihuili Hospital), The Affiliated Lihuili Hospital of Ningbo University, Ningbo, China (Yao, Li, Hu, Huang, Lv, Bu, Shen) School of Medicine, Ningbo University, Ningbo, China (Teh) Department of Ear, Nose, Throat-Head and Neck Surgery, Eastern Health, Box Hill, Australia (Teh) Department of Otolaryngology-Head and Neck Surgery, Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia (Zheng) Medical School, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Australiaen
dc.description.addressY. Shen, Department of Otolaryngology-Head and Neck Surgery, Ningbo Medical Center, Ningbo Lihuili Hospital), The Affiliated Lihuili Hospital of Ningbo University, Ningbo, China. E-mail: tyzdhs@163.com Y. Shen, School of Medicine, Ningbo University, Ningbo, China. E-mail: tyzdhs@163.comen
dc.subject.keywordhistopathologyen
dc.subject.keywordimmunohistochemistryen
dc.subject.keywordmaleen
dc.subject.keywordmyringoplastyen
dc.subject.keywordnonhumanen
dc.subject.keywordoptical coherence tomographyen
dc.subject.keywordotoscopyen
dc.subject.keyword*perforationen
dc.subject.keywordprospective studyen
dc.subject.keywordraten
dc.subject.keywordscanning electron microscopyen
dc.subject.keywordtissue regenerationen
dc.subject.keywordtransmission electron microscopyen
dc.subject.keywordtympanometryen
dc.subject.keywordtympanoplastyen
dc.subject.keywordultrastructureen
dc.subject.keywordwound healingen
dc.subject.keywordanimal tissueen
dc.subject.keywordanimal experimenten
dc.subject.keywordanimal modelen
dc.subject.keywordArticleen
dc.subject.keywordbrain stem responseen
dc.subject.keywordcontrolled studyen
dc.subject.keywordevoked brain stem auditory responseen
dc.subject.keywordevoked brain stem responseen
dc.subject.keyword*fibroblasten
dc.subject.keywordhearingen
dc.subject.keywordhistologyen
dc.relation.libraryurlLibKey Linken
dc.description.publicationstatusEmbaseen
dc.rights.statementCopyright 2021 Elsevier B.V., All rights reserved.en
dc.subect.keywordsbasic fibroblast growth factor collagen healing hearing scaffolds tympanic membrane perforationen
dc.identifier.authoremailShen Y.; tyzdhs@163.comen
dc.description.grantNo: 2018A610363 Organization: *Natural Science Foundation of Ningbo* Organization No: 100007834 Country: China No: 2018A610363 Organization: *Natural Science Foundation of Ningbo Municipality* Organization No: 501100005315 Country: China No: 81670920 Organization: (NSFC, NNSF, NNSFC) *National Natural Science Foundation of China* Organization No: 501100001809 Country: China No: LY15H130003 Organization: (ZJNSF) *Natural Science Foundation of Zhejiang Province* Organization No: 501100004731 Country: Chinaen
dc.identifier.affiliationext(Yao, Li, Hu, Huang, Lv, Shen) Department of Otolaryngology-Head and Neck Surgery, Ningbo Medical Center, Ningbo Lihuili Hospital), The Affiliated Lihuili Hospital of Ningbo University, Ningbo, China-
dc.identifier.affiliationext(Yao, Li, Hu, Huang, Lv, Bu, Shen) School of Medicine, Ningbo University, Ningbo, China-
dc.identifier.affiliationext(Teh) Department of Ear, Nose, Throat-Head and Neck Surgery, Eastern Health, Box Hill, Australia-
dc.identifier.affiliationext(Zheng) Medical School, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Australia-
dc.identifier.affiliationmh(Teh) Department of Otolaryngology-Head and Neck Surgery, Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia-
item.openairetypeArticle-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
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