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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Quinn T.A. | en |
dc.contributor.author | Walker D.W. | en |
dc.contributor.author | Nguyen T.-H. | en |
dc.contributor.author | Dickinson H. | en |
dc.contributor.author | Conley A.J. | en |
dc.contributor.author | Ratnayake U. | en |
dc.contributor.author | Castillo-Melendez M. | en |
dc.contributor.author | McIntosh M. | en |
dc.date.accessioned | 2021-05-14T09:15:28Z | en |
dc.date.available | 2021-05-14T09:15:28Z | en |
dc.date.copyright | 2013 | en |
dc.date.created | 20130320 | en |
dc.date.issued | 2013-03-20 | en |
dc.identifier.citation | Endocrinology. 154 (3) (pp 1190-1201), 2013. Date of Publication: 01 Mar 2013. | en |
dc.identifier.issn | 0013-7227 | en |
dc.identifier.uri | https://repository.monashhealth.org/monashhealthjspui/handle/1/27499 | en |
dc.description.abstract | Synthesis of the androgen dehydroepiandrosterone (DHEA) by the fetal adrenal gland is important for placental estrogen production and may also be important for modulating the effects of glucocorticoids on the developing brain. The presence of cortisol in spiny mouse (Acomys cahirinus) blood led us to determine whether the adrenal gland of this precocial rodent also synthesized DHEA. Cytochrome P450 enzyme 17alpha-hydroxylase/17,20-lyase (P450c17), cytochrome-b5 (Cytb5), and 3beta-hydroxysteroid dehydrogenase (3betaHSD) were detected in the adrenal gland from 30 days gestation (term=39 days), and DHEA, cortisol, and aldosterone were detected in fetal plasma from this time. PlasmaDHEAconcentrations increased 4-fold, whereas cortisol concentrations decreased from day 30 of gestation until the day of birth. Explant culture of fetal adrenal tissue showed that DHEA was produced from exogenous pregnenolone, and thus, the DHEA in the fetal circulation is likely to be of fetal origin. Clear zonation of the fetal adrenal cortex was evident by 38 days gestation when expression of Cytb5 was present throughout the cortex, and coexpression of P450c17 and Cytb5 occurred in the zona reticularis and fasciculata. 3betaHSD was expressed in the cortex from at least 30 days gestation and decreased as term approached, consistent with the fall of cortisol in late gestation in this species. These results show that the spiny mouse adrenal gland, like that of the human fetus, can synthesize and secrete DHEA from at least 30 days (relative gestation length, 30 days of a 39-day gestation, 0.76) of gestation, and DHEA may have important roles in placental biosynthesis of estrogens and in modulating the actions of glucocorticoids in the developing brain in this species. Copyright © 2013 by The Endocrine Society. | en |
dc.language | English | en |
dc.language | en | en |
dc.publisher | Endocrine Society (8401 Connecticut Ave. Suite 900, Chevy Chase MD 20815, United States) | en |
dc.subject | immunoblotting | en |
dc.subject | immunocytochemistry | en |
dc.subject | immunohistochemistry | en |
dc.subject | immunoreactivity | en |
dc.subject | maternal plasma | en |
dc.subject | mouse | en |
dc.subject | nonhuman | en |
dc.subject | *ontogeny | en |
dc.subject | perinatal period | en |
dc.subject | pregnancy | en |
dc.subject | prenatal period | en |
dc.subject | priority journal | en |
dc.subject | protein expression | en |
dc.subject | zona fasciculata | en |
dc.subject | zona glomerulosa | en |
dc.subject | zonation | en |
dc.subject | 3(or 17)beta hydroxysteroid dehydrogenase/ec [Endogenous Compound] | en |
dc.subject | aldosterone/ec [Endogenous Compound] | en |
dc.subject | corticosterone/ec [Endogenous Compound] | en |
dc.subject | cytochrome b5/ec [Endogenous Compound] | en |
dc.subject | *hydrocortisone/ec [Endogenous Compound] | en |
dc.subject | neurofilament protein/ec [Endogenous Compound] | en |
dc.subject | *prasterone/ec [Endogenous Compound] | en |
dc.subject | pregnenolone | en |
dc.subject | *steroid/ec [Endogenous Compound] | en |
dc.subject | steroid 17alpha monooxygenase/ec [Endogenous Compound] | en |
dc.subject | synaptophysin/ec [Endogenous Compound] | en |
dc.subject | tyrosine 3 monooxygenase/ec [Endogenous Compound] | en |
dc.subject | cell differentiation | en |
dc.subject | adrenal cortex | en |
dc.subject | *adrenal gland | en |
dc.subject | adrenal medulla | en |
dc.subject | aldosterone blood level | en |
dc.subject | animal cell | en |
dc.subject | animal experiment | en |
dc.subject | animal model | en |
dc.subject | animal tissue | en |
dc.subject | article | en |
dc.subject | biosynthesis | en |
dc.subject | controlled study | en |
dc.subject | corticosterone blood level | en |
dc.subject | explant | en |
dc.subject | female | en |
dc.subject | fetus | en |
dc.subject | fetus circulation | en |
dc.subject | gene expression | en |
dc.subject | gestational age | en |
dc.subject | hydrocortisone blood level | en |
dc.title | Ontogeny of the adrenal gland in the spiny mouse, with particular reference to production of the steroids cortisol and dehydroepiandrosterone. | en |
dc.type | Article | en |
dc.identifier.doi | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1210/en.2012-1953 | en |
dc.publisher.place | United States | en |
dc.identifier.pubmedid | 23354096 [http://www.ncbi.nlm.nih.gov/pubmed/?term=23354096] | en |
dc.identifier.source | 368462116 | en |
dc.identifier.institution | (Quinn, Ratnayake, Dickinson, Castillo-Melendez, Walker) Ritchie Centre, Monash Institute of Medical Research, Clayton, 3168, Australia (Walker) Department of Obstetrics and Gynaecology, Monash Medical Centre, Monash University, Clayton, 3168, Australia (Nguyen, McIntosh) Monash Institute of Pharmaceutical Sciences, Monash University, Parkville 3052, Australia (Conley) School of Veterinary Medicine, University of California Davis, Davis, CA 95616, United States | en |
dc.description.address | T.A. Quinn, Ritchie Centre, Monash Institute of Medical Research, Clayton, 3168, Australia | en |
dc.subject.keyword | controlled study | en |
dc.subject.keyword | corticosterone blood level | en |
dc.subject.keyword | explant | en |
dc.subject.keyword | female | en |
dc.subject.keyword | fetus | en |
dc.subject.keyword | fetus circulation | en |
dc.subject.keyword | gene expression | en |
dc.subject.keyword | gestational age | en |
dc.subject.keyword | hydrocortisone blood level | en |
dc.subject.keyword | immunoblotting | en |
dc.subject.keyword | immunocytochemistry | en |
dc.subject.keyword | immunohistochemistry | en |
dc.subject.keyword | immunoreactivity | en |
dc.subject.keyword | maternal plasma | en |
dc.subject.keyword | mouse | en |
dc.subject.keyword | nonhuman | en |
dc.subject.keyword | *ontogeny | en |
dc.subject.keyword | perinatal period | en |
dc.subject.keyword | pregnancy | en |
dc.subject.keyword | prenatal period | en |
dc.subject.keyword | priority journal | en |
dc.subject.keyword | protein expression | en |
dc.subject.keyword | zona fasciculata | en |
dc.subject.keyword | zona glomerulosa | en |
dc.subject.keyword | zonation | en |
dc.subject.keyword | *adrenal gland | en |
dc.subject.keyword | adrenal medulla | en |
dc.subject.keyword | adrenal cortex | en |
dc.subject.keyword | aldosterone blood level | en |
dc.subject.keyword | animal cell | en |
dc.subject.keyword | animal experiment | en |
dc.subject.keyword | animal model | en |
dc.subject.keyword | animal tissue | en |
dc.subject.keyword | article | en |
dc.subject.keyword | biosynthesis | en |
dc.subject.keyword | cell differentiation | en |
dc.relation.libraryurl | LibKey Link | en |
dc.description.publicationstatus | Embase | en |
dc.rights.statement | Copyright 2013 Elsevier B.V., All rights reserved. | en |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
Appears in Collections: | Articles |
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