Please use this identifier to cite or link to this item:
https://repository.monashhealth.org/monashhealthjspui/handle/1/29467
Conference/Presentation Title: | An in vitro assessment of the effect of intravenous lipid emulsion on total drug concentrations in human plasma utilising drugs commonly implicated in cardiovascular system poisoning. | Authors: | Alimovski Q.;Beyer J.;Graudins A. | Monash Health Department(s): | Emergency Medicine Clinical Toxicology |
Institution: | (Alimovski, Graudins) Southern Clinical School, Department of Pharmacology, Monash University, Clayton, VIC, Australia (Graudins) Emergency Medicine and Clinical Toxicology, Southern Health, Melbourne, VIC, Australia (Beyer) Victorian Institute, Forensic Medicine, Southbank, Melbourne, VIC, Australia | Presentation/Conference Date: | 24-Oct-2013 | Copyright year: | 2012 | Publisher: | Informa Healthcare | Publication information: | Clinical Toxicology. Conference: 2012 International Congress of the European Association of Poisons Centres and Clinical Toxicologists, EAPCCT 2012. London United Kingdom. Conference Publication: (var.pagings). 50 (4) (pp 339), 2012. Date of Publication: April 2012. | Abstract: | Background: The most commonly suggested mechanism of action for lipid emulsion (ILE) in poisoning is that it acts as an intravascular 'lipid-sink', allowing lipophilic drugs to bind with lipid molecules, reducing free plasma-drug concentrations. Little data exist assessing the interaction of ILE with drugs of varying lipid solubility. Objective(s): To assess the in vitro effect of ILE on total drug concentrations in human plasma exposed to various drugs implicated in cardiovascular system (CVS) poisoning and relate the findings to octanol:water coefficients of the drugs tested (LogP(octanol)). Method(s): Human plasma (1 mL samples) containing either amitriptyline, dothiepin, or clomipramine, verapamil or diltiazem, propranolol or atenolol, was incubated with 20% ILE (20, 100, 200 microlitres/mL) or equal volumes of phosphatebuffered saline (Control) in triplicate. Samples were centrifuged and the drug concentration in ILE-free plasma was assayed by GC/MS. Differences in drug concentrations between ILE and Control samples were expressed as percent-decrease ( +/- 95% CI) from Control. Unpaired t-test was used to compare drug concentrations between ILE samples and their respective Controls. Result(s): There were statistically significant decreases in ILE-treated plasma drug concentrations for the cyclic antidepressants and calcium channel-blockers. However, neither beta-blocker showed a significant decrease in drug concentrations at any ILE dose. Plasma drug concentrations fell more with increasing ILE concentration (See Table 1). Conclusion(s): A higher LogP(octanol) appears to correlate with in vitro reduction in plasma drug concentrations in the presence of ILE. Propranolol and atenolol, with the lowest LogP(octanol), did not exhibit significant sequestration into ILE. This technique may be a simple way to screen drugs for their potential binding to ILE. (Table Presented). | Conference Start Date: | 2012-05-25 | Conference End Date: | 2012-06-01 | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.3109/15563650.2012.669957 | ISSN: | 1556-3650 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/29467 | Type: | Conference Abstract |
Appears in Collections: | Conferences |
Show full item record
Items in Monash Health Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.