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DC Field | Value | Language |
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dc.contributor.author | Hurst L.A. | en |
dc.contributor.author | Atkins R.C. | en |
dc.contributor.author | Chadban S.J. | en |
dc.contributor.author | Nikolic-Paterson D.J. | en |
dc.contributor.author | Robertson T.E. | en |
dc.date.accessioned | 2021-05-14T11:12:29Z | en |
dc.date.available | 2021-05-14T11:12:29Z | en |
dc.date.copyright | 2002 | en |
dc.date.created | 20021119 | en |
dc.date.issued | 2012-10-18 | en |
dc.identifier.citation | Clinical and Experimental Immunology. 130 (2) (pp 241-244), 2002. Date of Publication: 2002. | en |
dc.identifier.issn | 0009-9104 | en |
dc.identifier.uri | https://repository.monashhealth.org/monashhealthjspui/handle/1/33055 | en |
dc.description.abstract | Interleukin-10 (IL-10) is a mesangial cell growth factor in vivo and in vitro. However, the mechanism by which IL-10 exerts its mitogenic activity is not known. The aim of this study was to determine whether IL-10 induces mesangial cell proliferation in a PDGF-dependent or independent fashion. A well-characterized rat mesangial cell line (1097) was used in a series of cell proliferation experiments in which cells were serum-starved and then incubated with recombinant IL-10 in the presence or absence of STI 571 (a specific inhibitor of signalling via the PDGF-alpha and beta receptors) or a neutralizing anti-PDGF-AB antibody. IL-10 induced significant mesangial cell proliferation at 24 and 48 h after cytokine addition. This response was inhibited totally by the addition of STI-571, demonstrating that IL-10 mitogenic activity has an absolute requirement for signalling through the PDGF receptor. In further studies, it was found that STI-571 could be added 24 h after IL-10 stimulation and still exert a profound inhibition of IL-10 mitogenic activity. The ability of a neutralizing anti-PDGF-AB antibody to inhibit completely IL-10-induced mesangial cell proliferation confirmed that IL-10 acts via induction of an autocrine PDGF response rather than the possibility that IL-10 may transactivate the PDGF receptor in a PDGF-independent fashion. In conclusion, this study has demonstrated that IL-10 induces mesangial cell proliferation via an autocrine PDGF-mediated mechanism. Thus, therapies which antagonize PDGF signalling will also inhibit any contribution of IL-10 to mesangial proliferation. | en |
dc.language | en | en |
dc.language | English | en |
dc.publisher | Blackwell Publishing Ltd (9600 Garsington Road, Oxford OX4 2XG, United Kingdom) | en |
dc.title | IL-10 induces mesangial cell proliferation via a PDGF-dependent mechanism. | en |
dc.type | Article | en |
dc.identifier.doi | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1046/j.1365-2249.2002.01975.x | en |
dc.publisher.place | United Kingdom | en |
dc.identifier.pubmedid | 12390311 [http://www.ncbi.nlm.nih.gov/pubmed/?term=12390311] | en |
dc.identifier.source | 35286210 | en |
dc.identifier.institution | (Robertson, Nikolic-Paterson, Hurst, Atkins, Chadban) Department of Nephrology, Monash Medical Centre, Clayton, Vic., Australia (Nikolic-Paterson, Atkins, Chadban) Monash University, Department of Medicine, Monash Medical Centre, Clayton, Vic., Australia (Chadban) Renal Medicine, Royal Prince Alfred Hospital, Missenden Road, Camperdown, NSW 2050, Australia | en |
dc.description.address | S.J. Chadban, Renal Medicine, Royal Prince Alfred Hospital, Missenden Road, Camperdown, NSW 2050, Australia. E-mail: steve.chadban@email.cs.nsw.gov.au | en |
dc.description.publicationstatus | Embase | en |
dc.rights.statement | Copyright 2012 Elsevier B.V., All rights reserved. | en |
dc.subect.keywords | Interleukin-10 Mesangial PDGF Proliferation STI-571 | en |
dc.identifier.authoremail | Chadban S.J.; steve.chadban@email.cs.nsw.gov.au | en |
item.fulltext | No Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | Article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | none | - |
crisitem.author.dept | Nephrology | - |
Appears in Collections: | Articles |
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