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Title: | Testosterone- and phorbol ester-stimulated proliferation in human cultured prostatic stromal cells. | Authors: | Frydenberg M. ;Majewski H.;Haynes J.M. | Institution: | (Haynes, Majewski) Department of Medical Laboratory Science, RMIT University, GPO Box 2476V, Melbourne, Vic. 3001, Australia (Frydenberg) Department of Urology, Monash Medical Centre, Moorabbin, Vic. 3189, Australia | Issue Date: | 23-Oct-2012 | Copyright year: | 2001 | Publisher: | Elsevier Inc. (360 Park Avenue South, New York NY 10010, United States) | Place of publication: | United States | Publication information: | Cellular Signalling. 13 (10) (pp 703-709), 2001. Date of Publication: 2001. | Abstract: | Prostatic stromal proliferation may be commonly associated with the development of benign prostatic hyperplasia. In this study, we investigate the role of testosterone and protein kinase C in stimulating cultured stromal cell proliferation. Testosterone increased the uptake of [3H]-thymidine into the human cultured prostatic stromal cells, this was reduced by the protein kinase C inhibitors, bisindolylymaleimide (10 nM) and myristoylated protein kinase C inhibitor (mPKCi, 20 muM), but not by Go 6983 (1 muM) or Go 6976 (1 muM). Cells responded to the addition of the PKC activators phorbol 12,13 dibutyrate (PDB), phorbol 12,13 diacetate (PDA), 12-deoxyphorbol 13-acetate (DPA) and 12-deoxyphorbol 13-tetradecanoate (DPT) with proliferation (order of potency DPT>=PDB>>PDA=DPA). The DPT-stimulated proliferative response was inhibited after cells were electroporated with PKCalpha antisense, but not mismatch oligonucleotides (8 muM). These results indicate that PKCalpha is involved in the proliferative response of human cultured prostatic stromal cells. © 2001 Elsevier Science Inc. All rights reserved. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1016/S0898-6568%2801%2900205-4 | PubMed URL: | 11602180 [http://www.ncbi.nlm.nih.gov/pubmed/?term=11602180] | ISSN: | 0898-6568 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/33141 | Type: | Article |
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