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DC Field | Value | Language |
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dc.contributor.author | Atkins R.C. | en |
dc.contributor.author | Tesch G.H. | en |
dc.contributor.author | Yang N. | en |
dc.contributor.author | Yu H. | en |
dc.contributor.author | Lan H.Y. | en |
dc.contributor.author | Foti R. | en |
dc.contributor.author | Chadban S.J. | en |
dc.contributor.author | Nikolic-Paterson D.J. | en |
dc.date.accessioned | 2021-05-14T11:27:48Z | en |
dc.date.available | 2021-05-14T11:27:48Z | en |
dc.date.copyright | 1997 | en |
dc.date.created | 19970616 | en |
dc.date.issued | 2012-10-22 | en |
dc.identifier.citation | Nephrology Dialysis Transplantation. 12 (6) (pp 1109-1115), 1997. Date of Publication: June 1997. | en |
dc.identifier.issn | 0931-0509 | en |
dc.identifier.uri | https://repository.monashhealth.org/monashhealthjspui/handle/1/33846 | en |
dc.description.abstract | Background. A number of studies have demonstrated a pathological role for interleukin-1 (IL-1) in experimental models of glomerulonephritis, but the cellular pattern of renal IL-1 production remains poorly characterized. The aim of this study, therefore, was to identify the cell types expressing IL-1 in normal and diseased rat kidney. Methods. Renal IL-1beta expression was examined in normal rats and during a 21-day time course of rat accelerated anti-GBM glomerulonephritis by northern blotting, in situ hybridization and double immunohistochemistry. Results. Interleukin-1beta mRNA expression was readily detectable in normal rat kidney by northern blot analysis and in situ hybridization. Immunohistochemistry staining demonstrated constitutive IL-1beta expression by glomerular endothelial cells and cortical tubular epithelial cells. There was a marked increase in whole kidney IL-1beta mRNA in rat anti-GBM glomerulonephritis. Glomerular IL-1beta immunostaining was upregulated, being expressed by podocytes, mesangial cells and infiltrating macrophages, and was particularly prominent within glomerular crescents. Double staining with the ED1 antibody showed IL-1beta expression in up to 13% of glomerular macrophages, whereas 48% of macrophages within crescents stained for IL-1beta. However, the most marked increase in IL-1beta expression was seen in cortical tubular epithelial cells, particularly in areas of tubular damage. In situ hybridization confirmed that tubular IL-1beta staining was due to local cytokine synthesis rather than protein absorption. Conclusions. This study has identified constitutive IL-1beta expression by glomerular endothelium and tubular epithelial cells in normal rat kidney. In addition, the marked upregulation of IL-1beta expression by intrinsic glomerular cells and tubules in rat anti-GBM disease suggests an important role for these cells in IL-1 dependent crescent formation and tubulointerstitial injury. | en |
dc.language | en | en |
dc.language | English | en |
dc.publisher | Oxford University Press (Great Clarendon Street, Oxford OX2 6DP, United Kingdom) | en |
dc.title | Intrinsic renal cells are the major source of interleukin-1beta synthesis in normal and diseased rat kidney. | en |
dc.type | Article | en |
dc.identifier.doi | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1093/ndt/12.6.1109 | en |
dc.publisher.place | United Kingdom | en |
dc.identifier.pubmedid | 9198037 [http://www.ncbi.nlm.nih.gov/pubmed/?term=9198037] | en |
dc.identifier.source | 27230543 | en |
dc.identifier.institution | (Tesch, Yang, Yu, Lan, Foti, Chadban, Atkins, Nikolic-Paterson) Department of Nephrology, Monash Medical Centre, Clayton, Vic., Australia (Nikolic-Paterson) Department of Nephrology, Monash Medical Centre, Clayton Road, Clayton, Vic. 3168, Australia | en |
dc.description.address | D.J. Nikolic-Paterson, Department of Nephrology, Monash Medical Centre, Clayton Road, Clayton, Vic. 3168, Australia | en |
dc.description.publicationstatus | Embase | en |
dc.rights.statement | Copyright 2012 Elsevier B.V., All rights reserved. | en |
dc.subect.keywords | Crescent formation Endothelium Glomerulonephritis Interleukin-1beta Macrophages Tubules | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairetype | Article | - |
crisitem.author.dept | Nephrology | - |
Appears in Collections: | Articles |
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