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https://repository.monashhealth.org/monashhealthjspui/handle/1/37541| Chapter/Book title: | An electrical impedance-based assay to examine functions of various placental cell types in vitro. | Authors: | Menkhorst E.;Narula S.;Sanderson J.T.;Murthi P.;Vaillancourt C.;Chollangi T.;Clabault H.;Thibeault A.-A.H.;Yong H.E.J. | Institution: | (Chollangi, Murthi) Department of Maternal-Fetal Medicine, Pregnancy Research Centre, The Royal Women's Hospital, Parkville, VIC, Australia (Chollangi) Department of Obstetrics and Gynecology, The University of Melbourne, Parkville, VIC, Australia (Clabault, Thibeault, Sanderson, Vaillancourt) INRS-Institut Armand-Frappier, 531 Boulevard des Prairies, Laval, QC H7V 1B7, Canada (Clabault, Thibeault) BioMed Research Centre, Universite du Quebec a Montreal, C.P. 8888, succ. Centre-ville, Montreal, QC H3C 3P8, Canada (Vaillancourt) BioMed Research Centre, Laval, QC, Canada (Yong) Department of Maternal-Fetal Medicine, Pregnancy Research Centre, The Royal Women's Hospital, Melbourne, VIC, Australia (Narula) The Ritchie Centre, Hudson Institute of Medical Research, Clayton, VIC, Australia (Menkhorst) Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, Australia (Murthi) Department of Obstetrics and Gynecology, The University of Melbourne, Clayton, VIC, Australia (Clabault, Thibeault, Vaillancourt) Center for Interdisciplinary Research on Well-Being, Health, Society and Environment (CINBIOSE), Universite du Quebec a Montreal, C.P. 8888, succ. Centre-ville, Montreal, QC H3C 3P8, Canada (Yong) Department of Obstetrics and Gynecology, The University of Melbourne, Melbourne, VIC, Australia (Yong) Centre for Trophoblast Research, Department of Physiology, Development and Neuroscience, The University of Cambridge, Cambridge, United Kingdom (Murthi) Department of Medicine, School of Clinical Sciences, Monash University, Level 5, R12, TRF, Monash Health, 27-31 Wright St, Clayton, VIC 3800, Australia | Publication year: | 2018 | Publisher: | Humana Press Inc. (E-mail: humana@humanapr.com) | Place of publication: | United States | Publication information: | Methods in Molecular Biology. 1710 (pp 267-276), 2018. Date of Publication: 2018. | Book title: | Methods in Molecular Biology | Abstract: | In vitro functional analyses of cells are widely used to investigate the molecular mechanisms involved in preeclampsia. Common cellular functions studied include adhesion, apoptosis, proliferation, migration, and invasion. At present, most researchers will use endpoint experimental assays that only allow the determination of cell function at a single time point, with the need to repeat the experiment for an alternate time point. Here, we describe an electrical impedance-based tool that allows real-time monitoring of cells, which enables the efficient assessment of multiple time points over the duration of a single experiment.Copyright © 2018, Springer Science+Business Media LLC. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1007/978-1-4939-7498-6_20 | PubMed URL: | 29197009 [http://www.ncbi.nlm.nih.gov/pubmed/?term=29197009] | ISSN: | 1064-3745 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/37541 | Type: | Chapter Chapter |
| Appears in Collections: | Books and Book Chapters |
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