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DC Field | Value | Language |
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dc.contributor.author | Sebire K. | en |
dc.contributor.author | Hedger M.P. | en |
dc.contributor.author | Gerdprasert O. | en |
dc.contributor.author | De Kretser D.M. | en |
dc.contributor.author | O'Bryan M.K. | en |
dc.contributor.author | Nikolic-Paterson D.J. | en |
dc.date.accessioned | 2021-05-14T09:02:19Z | en |
dc.date.available | 2021-05-14T09:02:19Z | en |
dc.date.copyright | 2002 | en |
dc.date.created | 20020722 | en |
dc.date.issued | 2002-07-22 | en |
dc.identifier.citation | Molecular Human Reproduction. 8 (6) (pp 518-524), 2002. Date of Publication: 2002. | en |
dc.identifier.issn | 1360-9947 | en |
dc.identifier.uri | https://repository.monashhealth.org/monashhealthjspui/handle/1/27016 | en |
dc.description.abstract | Macrophages are numerous in the testicular interstitial tissue under normal conditions and increase during inflammation. The mechanisms involved are poorly characterized. Expression of the macrophage-regulating cytokines monocyte chemoattractant protein (MCP)-1 and macrophage colony-stimulating factor (M-CSF) was examined in the adult rat testis before and after an i.p. injection of an inflammatory stimulus, lipopolysaccharide (LPS). In the normal testis, M-CSF was readily observed using Northern blot and Western blot analysis. In contrast, MCP-1 was not detectable by Northern blot in the normal testis, but was detected using RT-PCR amplification and a sensitive ELISA. After LPS treatment, testicular MCP-1 mRNA and protein expression increased dramatically (up to 400-fold). In-situ hybridization for MCP-1 revealed that production was confined to the interstitium of the inflamed testis, in Leydig cells, peritubular cells, perivascular cells and monocyte-like macrophages, but not in tissue-resident macrophages. Unlike MCP-1, M-CSF mRNA and protein expression in the testis increased only marginally, if at all, after LPS treatment. These results suggest that MCP-1 stimulates the increase in intratesticular macrophages that accompanies LPS-induced inflammation in vivo. Together with M-CSF, MCP-1 may also play a role in maintaining the resident macrophage population of the normal testis. | en |
dc.language | en | en |
dc.language | English | en |
dc.publisher | Oxford University Press | en |
dc.publisher | Oxford University Press (Great Clarendon Street, Oxford OX2 6DP, United Kingdom) | en |
dc.relation.ispartof | Molecular Human Reproduction | en |
dc.title | Expression of monocyte chemoattractant protein-1 and macrophage colony-stimulating factor in normal and inflamed rat testis. | en |
dc.type | Article | en |
dc.identifier.doi | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1093/molehr/8.6.518 | en |
dc.publisher.place | United Kingdom | en |
dc.identifier.pubmedid | 12029068 [http://www.ncbi.nlm.nih.gov/pubmed/?term=12029068] | en |
dc.identifier.source | 34746284 | en |
dc.identifier.institution | (Gerdprasert, O'Bryan, Sebire, De Kretser, Hedger) Monash Institute of Reproduction and Development, Monash University, 27-31 Wright Street, Clayton, Vic., Australia (Nikolic-Paterson) Department of Nephrology, Monash Medical Centre, Clayton 3168, Vic., Australia (Nikolic-Paterson) Monash University Department of Medicine, Monash Medical Centre, Clayton 3168, Vic., Australia | en |
dc.description.address | M.P. Hedger, Monash Inst. of Reproduction/Devt., Monash University, Monash Medical Centre, 27-31 Wright Street, Clayton, Vic. 3168, Australia. E-mail: mark.hedger@med.monash.edu.au | en |
dc.description.publicationstatus | Embase | en |
dc.rights.statement | Copyright 2012 Elsevier B.V., All rights reserved. | en |
dc.rights.statement | Copyright 2021 Elsevier B.V., All rights reserved. | en |
dc.subect.keywords | Inflammation M-CSF MCP-1 Monocytes Testis | en |
dc.identifier.authoremail | Hedger M.P.; mark.hedger@med.monash.edu.au | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.fulltext | No Fulltext | - |
item.openairetype | Article | - |
crisitem.author.dept | Nephrology | - |
Appears in Collections: | Articles |
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