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Title: | Expression of monocyte chemoattractant protein-1 and macrophage colony-stimulating factor in normal and inflamed rat testis. | Authors: | Sebire K.;Hedger M.P.;Gerdprasert O.;De Kretser D.M.;O'Bryan M.K.;Nikolic-Paterson D.J. | Institution: | (Gerdprasert, O'Bryan, Sebire, De Kretser, Hedger) Monash Institute of Reproduction and Development, Monash University, 27-31 Wright Street, Clayton, Vic., Australia (Nikolic-Paterson) Department of Nephrology, Monash Medical Centre, Clayton 3168, Vic., Australia (Nikolic-Paterson) Monash University Department of Medicine, Monash Medical Centre, Clayton 3168, Vic., Australia | Issue Date: | 22-Jul-2002 | Copyright year: | 2002 | Publisher: | Oxford University Press Oxford University Press (Great Clarendon Street, Oxford OX2 6DP, United Kingdom) |
Place of publication: | United Kingdom | Publication information: | Molecular Human Reproduction. 8 (6) (pp 518-524), 2002. Date of Publication: 2002. | Journal: | Molecular Human Reproduction | Abstract: | Macrophages are numerous in the testicular interstitial tissue under normal conditions and increase during inflammation. The mechanisms involved are poorly characterized. Expression of the macrophage-regulating cytokines monocyte chemoattractant protein (MCP)-1 and macrophage colony-stimulating factor (M-CSF) was examined in the adult rat testis before and after an i.p. injection of an inflammatory stimulus, lipopolysaccharide (LPS). In the normal testis, M-CSF was readily observed using Northern blot and Western blot analysis. In contrast, MCP-1 was not detectable by Northern blot in the normal testis, but was detected using RT-PCR amplification and a sensitive ELISA. After LPS treatment, testicular MCP-1 mRNA and protein expression increased dramatically (up to 400-fold). In-situ hybridization for MCP-1 revealed that production was confined to the interstitium of the inflamed testis, in Leydig cells, peritubular cells, perivascular cells and monocyte-like macrophages, but not in tissue-resident macrophages. Unlike MCP-1, M-CSF mRNA and protein expression in the testis increased only marginally, if at all, after LPS treatment. These results suggest that MCP-1 stimulates the increase in intratesticular macrophages that accompanies LPS-induced inflammation in vivo. Together with M-CSF, MCP-1 may also play a role in maintaining the resident macrophage population of the normal testis. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1093/molehr/8.6.518 | PubMed URL: | 12029068 [http://www.ncbi.nlm.nih.gov/pubmed/?term=12029068] | ISSN: | 1360-9947 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/27016 | Type: | Article |
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