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https://repository.monashhealth.org/monashhealthjspui/handle/1/37350| Chapter/Book title: | A comprehensive protocol resource for performing pooled shRNA and CRISPR screens. | Authors: | Arnau G.M.;Cowley K.J.;Semple T.;Shortt, Jake ;Simpson K.J.;Cluse L.A.;Nikolic I.;Knight D.;Johnstone R.W.;Madhamshettiwar P.B.;Luu J. | Institution: | (Cluse, Knight, Johnstone) Gene Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia (Nikolic, Madhamshettiwar, Luu, Cowley, Simpson) Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia (Semple, Arnau) Molecular Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia (Shortt) School of Clinical Sciences at Monash Health, Monash University, Clayton, VIC, Australia (Johnstone, Simpson) Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, Australia | Publication year: | 2018 | Publisher: | Humana Press Inc. (E-mail: humana@humanapr.com) | Place of publication: | United States | Publication information: | Methods in Molecular Biology. 1725 (pp 201-227), 2018. Date of Publication: 2018. | Book title: | Methods in Molecular Biology | Abstract: | This chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen-with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories.Copyright © 2018, Springer Science+Business Media, LLC. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1007/978-1-4939-7568-6_17 | PubMed URL: | 29322420 [http://www.ncbi.nlm.nih.gov/pubmed/?term=29322420] | ISSN: | 1064-3745 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/37350 | Type: | Chapter Chapter |
| Appears in Collections: | Books and Book Chapters |
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