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Title: | Effect of storage duration on cytokine stability in human serum and plasma. | Authors: | Lee J.P.W.;Harris J.;Morand E.F. ;Vincent F.B. ;Nim H.T. | Institution: | (Vincent, Lee, Morand, Harris) Rheumatology Research Group, Centre for Inflammatory Diseases, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing & Health Sciences, Monash University, Clayton, Victoria 3168, Australia (Nim) Faculty of Information Technology, Monash University, Clayton, Victoria 3800, Australia (Nim) Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria 3800, Australia | Issue Date: | 12-Dec-2018 | Copyright year: | 2019 | Publisher: | Academic Press | Place of publication: | United Kingdom | Publication information: | Cytokine. 113 (pp 453-457), 2019. Date of Publication: January 2019. | Journal: | Cytokine | Abstract: | Quantification of analytes such as cytokines in serum samples is intrinsic to translational research in immune diseases. Optimising pre-analytical conditions is critical for ensuring study quality, including evaluation of cytokine stability. We aimed to evaluate the effect on cytokine stability of storage duration prior to freezing of serum, and compare to plasma samples obtained from patients with systemic lupus erythematosus (SLE). Protein stability was analysed by simultaneously quantifying 18 analytes using a custom multi-analyte profile in SLE patient serum and plasma samples that had been prospectively stored at 4 degreeC for pre-determined periods between 0 and 30 days, prior to freezing. Six analytes were excluded from analysis, because most tested samples were above or below the limit of detection. Amongst the 12 analysed proteins, 11 did not show significant signal degradation. Significant signal degradation was observed from the fourth day of storage for a single analyte, CCL19. Proteins levels were more stable in unseparated serum compared to plasma for most analytes, with the exception of IL-37 which appears slightly more stable in plasma. Based on this, a maximum 3 days of storage at 4 degreeC for unseparated serum samples is recommended for biobanked samples intended for cytokine analysis in studies of human immune disease.Copyright © 2018 Elsevier Ltd | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1016/j.cyto.2018.06.009 | Link to associated publication: | Click here for full text options | PubMed URL: | 29909979 [http://www.ncbi.nlm.nih.gov/pubmed/?term=29909979] | ISSN: | 1043-4666 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/37488 | Type: | Article | Subjects: | interleukin 1 receptor type I/ec [Endogenous Compound] interleukin 1 receptor type II/ec [Endogenous Compound] interleukin 10/ec [Endogenous Compound] interleukin 17/ec [Endogenous Compound] interleukin 18/ec [Endogenous Compound] interleukin 1alpha/ec [Endogenous Compound] interleukin 1beta/ec [Endogenous Compound] interleukin 33/ec [Endogenous Compound] interleukin 37/ec [Endogenous Compound] interleukin 6/ec [Endogenous Compound] leptin/ec [Endogenous Compound] macrophage inflammatory protein 3beta/ec [Endogenous Compound] monocyte chemotactic protein 1/ec [Endogenous Compound] tumor necrosis factor/ec [Endogenous Compound] interleukin 1 receptor blocking agent/ec [Endogenous Compound] adult article blood sampling clinical article controlled study female freezing human plasma priority journal protein analysis *protein stability serum *storage systemic lupus erythematosus *cytokine/ec [Endogenous Compound] gamma interferon/ec [Endogenous Compound] systemic lupus erythematosus controlled study clinical article *protein stability blood sampling protein analysis Article adult priority journal female *storage plasma human freezing serum |
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