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Title: | Resveratrol inhibits release of soluble fms-like tyrosine kinase (sFlt-1) and soluble endoglin and improves vascular dysfunction - implications as a preeclampsia treatment. | Authors: | Nguyen T.V.;Palmer, Kirsten R. ;Tong S.;Kaitu'u-Lino T.J.;Hannan N.J.;Brownfoot F.C.;Cannon P.;Deo M.;Beard S. | Institution: | (Hannan, Brownfoot, Cannon, Deo, Beard, Nguyen, Tong) Translational Obstetrics Group, The Department of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, 163 Studley Rd, Heidelberg, 3084, Victoria, Australia (Palmer) Department of Obstetrics and Gynaecology, Monash University, Monash Medical Centre, 246 Clayton Rd ,Clayton, Victoria 3168, Australia (Kaitu'u-Lino) Translational Obstetrics Group, The Department of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, 163 Studley Rd, Heidelberg, 3084, Victoria, Australia. t.klino@unimelb.edu.au | Issue Date: | 17-Nov-2018 | Copyright year: | 2017 | Publisher: | NLM (Medline) | Place of publication: | United Kingdom | Publication information: | Scientific reports. 7 (1) (pp 1819), 2017. Date of Publication: 12 May 2017. | Abstract: | Preeclampsia is a disease of pregnancy associated with placental oxidative stress, inflammation and elevated release of anti-angiogenic factors sFlt-1 and soluble endoglin. These placental factors cause generalized maternal endothelial dysfunction. There are no treatments to halt disease progression; delivery is the only cure. Resveratrol modulates pathways involved in inflammation and oxidative stress and may offer a potential therapeutic for preeclampsia. Resveratrol reduced sFlt-1, sFlt-1 e15a and soluble endoglin secretion from primary trophoblasts and HUVECs and reduced mRNA expression of pro-inflammatory molecules NFkappaB, IL-6 and IL-1beta in trophoblasts. IL-6, IL-1beta and TNFalpha secretion were also significantly reduced. In HUVECs, resveratrol significantly increased mRNA of anti-oxidant enzymes HO-1, NQO1, GCLC and TXN but did not significantly alter HO-1 protein expression, whilst reducing HO-1 protein in trophoblast. Endothelial dysfunction was induced in HUVECs using TNFalpha, increasing expression of cell adhesion molecule VCAM1 and adhesion of peripheral blood mononuclear cells, both of which were increased further by resveratrol. In contrast, resveratrol significantly reduced TNFalpha-induced Endothelin-1 (a vasoconstrictor) and significantly increased the phosphorylation of endothelial nitric oxide synthase (eNOS). In summary, resveratrol decreases secretion of anti-angiogenic factors however its effects on the endothelium are mixed. Overall, it may have potential as a treatment for preeclampsia. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1038/s41598-017-01993-w | Link to associated publication: | Click here for full text options | PubMed URL: | 28500309 [http://www.ncbi.nlm.nih.gov/pubmed/?term=28500309] | ISSN: | 2045-2322 (electronic) | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/38594 | Type: | Article | Subjects: | heme oxygenase 1 resveratrol/pd [Pharmacology] resveratrol/dt [Drug Therapy] vasculotropin receptor 1 *antagonists and inhibitors FLT1 protein, human preeclampsia/et [Etiology] *biosynthesis blood vessel cell survival *drug effect female genetics human metabolism oxidative stress preeclampsia/dt [Drug Therapy] pregnancy trophoblast umbilical vein endothelial cell vascular endothelium antioxidant/pd [Pharmacology] autacoid biological marker cytokine endoglin endothelial nitric oxide synthase umbilical vein endothelial cell vascular endothelium *drug effect cell survival pregnancy blood vessel preeclampsia / drug therapy / etiology *biosynthesis oxidative stress metabolism female genetics trophoblast human |
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