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Title: | The effect of exercise-intensity on Skeletal muscle stress kinase and insulin protein signaling. | Authors: | Shaw C.S.;Parker L.;Trewin A.;Levinger I.;Stepto N.K. | Institution: | (Parker, Trewin, Levinger, Shaw, Stepto) Institute of Sport, Exercise and Active Living (ISEAL), College of Sport and Exercise Science, Victoria University, Melbourne, Australia (Shaw) Institute for Physical Activity and Nutrition, School of Exercise and Nutrition Sciences, Deakin University, Geelong, Australia (Stepto) Monash Centre for Health Research and Implementation (MCHRI), School of Public Health and Preventative Medicine, Monash University and Monash Health, Clayton, Australia | Issue Date: | 24-Feb-2017 | Copyright year: | 2017 | Publisher: | Public Library of Science (E-mail: plos@plos.org) | Place of publication: | United States | Publication information: | PLoS ONE. 12 (2) (no pagination), 2017. Article Number: e0171613. Date of Publication: February 2017. | Journal: | PLoS ONE | Abstract: | Background: Stress and mitogen activated protein kinase (SAPK) signaling play an important role in glucose homeostasis and the physiological adaptation to exercise. However, the effects of acute high-intensity interval exercise (HIIE) and sprint interval exercise (SIE) on activation of these signaling pathways are unclear. Method(s): Eight young and recreationally active adults performed a single cycling session of HIIE (5x4 minutes at 75% Wmax), SIE (4 x 30 second Wingate sprints), and continuous moderate-intensity exercise work-matched to HIIE (CMIE; 30 minutes at 50% of Wmax), separated by a minimum of 1 week. Skeletal muscle SAPK and insulin protein signaling were measured immediately, and 3 hours after exercise. Result(s): SIE elicited greater skeletal muscle NF-kappaB p65 phosphorylation immediately after exercise (SIE: ~40%; HIIE: ~4%; CMIE; ~13%; p < 0.05) compared to HIIE and CMIE. AS160Ser588 phosphorylation decreased immediately after HIIE (~-27%; p < 0.05), and decreased to the greatest extent immediately after SIE (~-60%; p < 0.05). Skeletal muscle JNK (~42%; p < 0.05) and p38 MAPK (~171%; p < 0.05) phosphorylation increased, and skeletal muscle AktSer473 phosphorylation (~-32%; p < 0.05) decreased, to a similar extent immediately after all exercise protocols. AS160Ser588 phosphorylation was similar to baseline three hours after SIE (~-12%; p > 0.05), remained lower 3 hours after HIIE (~-34%; p < 0.05), and decreased 3 hours after CMIE (~-33%; p < 0.05). Conclusion(s): Despite consisting of less total work than CMIE and HIIE, SIE proved to be an effective stimulus for the activation of stress protein kinase signaling pathways linked to exercise-mediated adaptation of skeletal muscle. Furthermore, post-exercise AS160Ser588 phosphorylation decreased in an exercise-intensity and post-exercise time-course dependent manner.Copyright © 2017 Parker et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1371/journal.pone.0171613 | Link to associated publication: | Click here for full text options | PubMed URL: | 28182793 [http://www.ncbi.nlm.nih.gov/pubmed/?term=28182793] | ISSN: | 1932-6203 (electronic) | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/39453 | Type: | Article | Subjects: | *mitogen activated protein kinase p38/ec [Endogenous Compound] adaptation adult article blood sampling cycling enzyme activation *exercise intensity female human human experiment male normal human protein analysis protein phosphorylation signal transduction skeletal muscle time volunteer glucose/ec [Endogenous Compound] lactic acid/ec [Endogenous Compound] *insulin/ec [Endogenous Compound] transcription factor RelA/ec [Endogenous Compound] *stress activated protein kinase/ec [Endogenous Compound] protein kinase B/ec [Endogenous Compound] enzyme activation *exercise intensity female human time skeletal muscle signal transduction protein phosphorylation human experiment volunteer adaptation adult Article blood sampling cycling protein analysis normal human male |
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