Please use this identifier to cite or link to this item:
https://repository.monashhealth.org/monashhealthjspui/handle/1/49625| Title: | Generation and multi-dimensional profiling of a childhood cancer cell line atlas defines new therapeutic opportunities. | Authors: | Sun C.X.;Daniel P.;Bradshaw G.;Shi H.;Loi M.;Chew N.;Parackal S.;Tsui V.;Liang Y.;Koptyra M.;Adjumain S.;Sun C.;Chong W.C.;Fernando D.;Drinkwater C.;Tourchi M.;Habarakada D.;Sooraj D.;Carvalho D.;Storm P.B.;Baubet V.;Sayles L.C.;Fernandez E.;Nguyen T.;Porksen M.;Doan A.;Crombie D.E.;Panday M.;Zhukova N.;Dun M.D.;Ludlow L.E.;Day B.;Stringer B.W.;Neeman N.;Rubens J.A.;Raabe E.H.;Vinci M.;Tyrrell V.;Fletcher J.I.;Ekert P.G.;Dumevska B.;Ziegler D.S.;Tsoli M.;Syed Sulaiman N.F.;Loh A.H.P.;Low S.Y.Y.;Sweet-Cordero E.A.;Monje M.;Resnick A.;Jones C.;Downie P. ;Williams B.;Rosenbluh J.;Gough D. ;Cain J.E.;Firestein R. | Monash Health Department(s): | Hudson Institute - Centre for Cancer Research Paediatric - Haematology-Oncology (Children's Cancer Centre) |
Institution: | (Sun, Daniel, Bradshaw, Shi, Loi, Chew, Parackal, Tsui, Liang, Adjumain, Sun, Chong, Fernando, Drinkwater, Tourchi, Habarakada, Sooraj, Nguyen, Porksen, Doan, Crombie, Panday, Zhukova, Neeman, Williams, Gough, Cain, Firestein) Centre for Cancer Research, Hudson Institute of Medical Research, Monash University, Clayton, VIC 3168, Australia (Sun, Daniel, Bradshaw, Shi, Loi, Chew, Parackal, Tsui, Liang, Adjumain, Sun, Chong, Fernando, Drinkwater, Tourchi, Habarakada, Sooraj, Nguyen, Porksen, Doan, Crombie, Panday, Zhukova, Neeman, Williams, Gough, Cain, Firestein) Department of Molecular and Translational Science, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, VIC 3168, Australia (Koptyra, Storm, Baubet, Resnick) Center for Data Driven Discovery in Biomedicine, Neurosurgery Department, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, United States (Carvalho, Fernandez, Jones) Division of Molecular Pathology, The Institute of Cancer Research, London SM2 5NG, United Kingdom (Carvalho, Fernandez, Jones) Division of Cancer Therapeutics, The Institute of Cancer Research, London SM2 5NG, United Kingdom (Sayles, Sweet-Cordero) Department of Pediatrics, University of California San Francisco, San Francisco, CA 94158, United States (Dun) Hunter Cancer Research Alliance, University of Newcastle, Callaghan, NSW 2308, Australia (Dun) School of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, NSW 2308, Australia (Ludlow) Children's Cancer Centre Biobank, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC 3052, Australia (Day, Stringer) QIMR Berghofer Medical Research Institute, Herston, QLD 4006, Australia (Rubens, Raabe) Division of Pediatric Oncology, Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, United States (Raabe) Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, United States (Vinci) Department of Onco-haematology, Cell and Gene Therapy, Bambino Gesu Children's Hospital-IRCCS, Rome 00165, Italy (Tyrrell, Fletcher, Ekert, Dumevska, Ziegler, Tsoli) Children's Cancer Institute, Lowy Cancer Research Centre, UNSW Sydney, Sydney, NSW, Australia (Tyrrell, Fletcher, Ekert, Ziegler, Tsoli) School of Clinical Medicine, UNSW Sydney, Sydney, NSW, Australia (Ekert) Centre for Cancer Immunotherapy, Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia (Ekert) Department of Oncology, Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia (Ekert) Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, VIC 3052, Australia (Ziegler) Kids Cancer Centre, Sydney Children's Hospital, Randwick, NSW 2031, Australia (Syed Sulaiman, Low) Neurosurgical Service, KK Women's and Children's Hospital, Singapore 229899, Singapore (Syed Sulaiman, Loh, Low) VIVA-KKH Paediatric Brain and Solid Tumours Programme, Singapore 229899, Singapore (Loh) Duke-NUS Medical School, Singapore 169857, Singapore (Low) SingHealth-Duke NUS Neuroscience Academic Clinical Programme, Singapore 308433, Singapore (Low) SingHealth-Duke NUS Paediatrics Academic Clinical Programme, Singapore 229899, Singapore (Monje) Department of Neurology, Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, United States (Zhukova, Downie) Children's Cancer Centre, Monash Children's Hospital, Monash Health, Clayton, VIC 3168, Australia (Zhukova, Downie) Department of Paediatrics, Monash University, Clayton, VIC 3168, Australia (Porksen) Department of Paediatrics, University of Lubeck, Lubeck 23562, Germany (Rosenbluh) Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3168, Australia |
Issue Date: | 10-Apr-2023 | Copyright year: | 2023 | Publisher: | Cell Press | Place of publication: | United States | Publication information: | Cancer Cell. 41(4) (pp 660-677.e7), 2023. Date of Publication: 10 Apr 2023. | Journal: | Cancer Cell | Abstract: | Pediatric solid and central nervous system tumors are the leading cause of cancer-related death among children. Identifying new targeted therapies necessitates the use of pediatric cancer models that faithfully recapitulate the patient's disease. However, the generation and characterization of pediatric cancer models has significantly lagged behind adult cancers, underscoring the urgent need to develop pediatric-focused cell line resources. Herein, we establish a single-site collection of 261 cell lines, including 224 pediatric cell lines representing 18 distinct extracranial and brain childhood tumor types. We subjected 182 cell lines to multi-omics analyses (DNA sequencing, RNA sequencing, DNA methylation), and in parallel performed pharmacological and genetic CRISPR-Cas9 loss-of-function screens to identify pediatric-specific treatment opportunities and biomarkers. Our work provides insight into specific pathway vulnerabilities in molecularly defined pediatric tumor classes and uncovers biomarker-linked therapeutic opportunities of clinical relevance. Cell line data and resources are provided in an open access portal.Copyright © 2023 Elsevier Inc. | DOI: | http://monash.idm.oclc.org/login?url=https://dx.doi.org/10.1016/j.ccell.2023.03.007 | PubMed URL: | 37001527 [https://www.ncbi.nlm.nih.gov/pubmed/?term=37001527] | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/49625 | Type: | Article | Subjects: | brain cancer DNA sequencing epigenetics multiomics RNA sequencing |
Type of Clinical Study or Trial: | Observational study (cohort, case-control, cross sectional, or survey) |
| Appears in Collections: | Articles |
Show full item record
Items in Monash Health Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.