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Title: | Acellular Collagen Scaffold With Basic Fibroblast Growth Factor for Repair of Traumatic Tympanic Membrane Perforation in a Rat Model. | Authors: | Huang J.;Teh B.M.;Li H.;Hu Y.;Shen Y.;Zheng M.;Bu S.;Lv C.;Yao X. | Institution: | (Yao, Li, Hu, Huang, Lv, Shen) Department of Otolaryngology-Head and Neck Surgery, Ningbo Medical Center, Ningbo Lihuili Hospital), The Affiliated Lihuili Hospital of Ningbo University, Ningbo, China (Yao, Li, Hu, Huang, Lv, Bu, Shen) School of Medicine, Ningbo University, Ningbo, China (Teh) Department of Ear, Nose, Throat-Head and Neck Surgery, Eastern Health, Box Hill, Australia (Teh) Department of Otolaryngology-Head and Neck Surgery, Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Australia (Zheng) Medical School, Faculty of Health and Medical Sciences, The University of Western Australia, Nedlands, Australia | Issue Date: | 6-Apr-2021 | Copyright year: | 2021 | Publisher: | SAGE Publications Inc. | Place of publication: | United States | Publication information: | Otolaryngology - Head and Neck Surgery (United States). 164 (2) (pp 381-390), 2021. Date of Publication: February 2021. | Journal: | Otolaryngology - Head and Neck Surgery | Abstract: | Objective: To evaluate the efficacy of acellular collagen scaffold (ACS) in combination with basic fibroblast growth factor (bFGF) for the repair of traumatic tympanic membrane (TM) perforation in a rat model. Study Design: A prospective controlled animal study in a rat model of traumatic TM perforation. Setting(s): Tertiary medical center. Subjects and Methods: Sprague-Dawley rats (N = 84) with unilateral traumatic perforation of the right TMs were randomized to receive ACS, bFGF, ACS in combination with bFGF (ACS/bFGF), or nothing (spontaneous healing without any interventions as a control group). The healing outcomes were evaluated by otoscopy, optical coherence tomography, histology, and transmission electron microscopy at 1, 2, and 4 weeks postoperatively. The hearing outcomes were assessed with auditory brainstem response testing. Result(s): ACS/bFGF resulted in higher perforation closure rates at an earlier stage than spontaneous healing, ACS, and bFGF. Based on histology, optical coherence tomography, and transmission electron microscopy, a trilaminar structure and uniform thickness with mature, densely packed collagen fibers were seen in the ACS/bFGF group. Auditory brainstem response evaluation also showed that ACS/bFGF treatment promoted faster functional hearing recovery as compared with the control group. Conclusion(s): ACS is an effective TM scaffold and a carrier for bFGF. ACS/bFGF improves the TM closure rate, results in better-reconstructed TMs, and improves hearing. ACS/bFGF serves as a potential substitute for TM perforations in clinical settings.Copyright © American Academy of Otolaryngology-Head and Neck Surgery Foundation 2020. | DOI: | http://monash.idm.oclc.org/login?url= http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1177/0194599820938345 |
PubMed URL: | 32662734 [http://www.ncbi.nlm.nih.gov/pubmed/?term=32662734] | ISSN: | 0194-5998 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/26770 | Type: | Article | Subjects: | tissue regeneration transmission electron microscopy tympanometry tympanoplasty ultrastructure wound healing *collagen collagen type 1 fibroblast growth factor fibroblast growth factor 2 *molecular scaffold otoscope Model 4000 TECNAI 10 *perforation animal experiment animal model animal tissue article brain stem response controlled study evoked brain stem auditory response evoked brain stem response *fibroblast hearing histology histopathology immunohistochemistry male myringoplasty nonhuman optical coherence tomography otoscopy prospective study rat scanning electron microscopy histopathology immunohistochemistry male myringoplasty nonhuman optical coherence tomography otoscopy *perforation prospective study rat scanning electron microscopy tissue regeneration transmission electron microscopy tympanometry tympanoplasty ultrastructure wound healing animal tissue animal experiment animal model Article brain stem response controlled study evoked brain stem auditory response evoked brain stem response *fibroblast hearing histology |
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