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Title: | Production of embryos from in vitro-matured primary human oocytes. | Authors: | Wood C.;Tiglias J.;Wilton L.;Trounson A.;Barnes F.L.;Kausche A. | Institution: | (Barnes, Kausche, Tiglias, Wood, Wilton, Trounson) Inst. of Reproduction/Development, Monash Medical Centre, 246 Clayton Road, Clayton, Vic. 3168, Australia | Issue Date: | 22-Oct-2012 | Copyright year: | 1996 | Publisher: | Elsevier Inc. Elsevier Inc. (360 Park Avenue South, New York NY 10010, United States) |
Place of publication: | United States | Publication information: | Fertility and Sterility. 65 (6) (pp 1151-1156), 1996. Date of Publication: 1996. | Journal: | Fertility and Sterility | Abstract: | Objective: To determine the factors that influence the number and quality of embryos produced from primary oocytes collected from untreated regularly ovulating and irregular or anovulatory polycystic women. Design(s): A direct comparison between two patient groups whose oocytes were matured in vitro and a comparison of the embryo development of in vitro-matured oocytes from untreated patients with in vivo-matured oocytes of superovulated IVF-ET patients obtained during the same period. Setting(s): The Monash IVF Clinic, involving patients who expressed the desire to avoid superovulation with fertility drugs. Main Outcome Measure(s): The completion of nuclear maturation of oocytes after 36 or 48 hours culture, fertilization in vitro, and embryo development ratio. Result(s): Oocytes from regular cycling patients matured and fertilized at significantly higher rates than irregular cycling and anovulatory women and their embryos had significantly higher mean embryo development ratio. The mean embryo development ratio of embryos of regular cycling patients was similar to superovulated IVF patients but irregular cycling and anovulatory patients had a significantly lower embryo development ratio. Culture of oocytes for 48 hours increased maturation of oocytes from 57% to 82% but did not affect fertilization or cleavage rates. Embryo development was not affected significantly by the grade of follicular cell cover of oocytes. Conclusion(s): The developmental capability of primary oocytes is higher in regular cycling women than in irregular cycling and anovulatory women with polycystic ovary disease. | DOI: | http://monash.idm.oclc.org/login?url=http://dx.doi.org/10.1016/s0015-0282%2816%2958330-7 | PubMed URL: | 8641489 [http://www.ncbi.nlm.nih.gov/pubmed/?term=8641489] | ISSN: | 0015-0282 | URI: | https://repository.monashhealth.org/monashhealthjspui/handle/1/26944 | Type: | Article | Subjects: | histopathology human human cell human tissue menstrual cycle *oocyte maturation *ovary polycystic disease/di [Diagnosis] priority journal treatment outcome clinical article embryo development article female fertilization in vitro anovulation/et [Etiology] anovulation / etiology priority journal *ovary polycystic disease / *diagnosis *oocyte maturation menstrual cycle female embryo development clinical article article treatment outcome human tissue human cell human histopathology fertilization in vitro |
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